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2021 NMR Users Workshop
Steps for Protein NMR Structure Determination
High-Field NMR Center & GRC NMR LAB
Academia Sinica
Jan. 27-29, 2021
！＊＊　Postpone　＊＊！
Time January 27th - 29th, 2021　( Postpone !! )　
Registration Deadline January 20th, 2021 (Wednesday) ... 逾期恕不受理，敬請配合 !!
Registration Link Registration ... over Deadline
Registration fee Free of charge
Place B1A & B1B & B1C & B2 HFNMRC, IBMS, Academia Sinica, Taipei, Taiwan R.O.C.
Course Language Chinese(major) / English (中文為主,英文助講)

Topic :

  • Useful Experiments for Protein NMR
  • Protein Production and Labeling
  • NMR Data Collection & Processing using Topspin
  • Protein Resonance Assignment using Sparky
  • Protein Structure Calculation using CYANA
  • Protein Structure Display using MOLMOL
  • Structure Visualization using PyMoL

Hands-On :

ps: 課程進行中將不支援軟體安裝等協助以避免影響其他學員的學習進度與時間。
  • NMR Data Collection ( in groups, 分組進行 )
  • NMR Data Processing ( 事前請先完成安裝: Topspin )
  • Protein Backbone Assignment ( 事前請先完成安裝: Sparky )
  • Protein Structure Calculation ( 事前請先完成安裝: Molmol & PuTTY )
  • Structure Visualization ( 事前請先完成安裝: PyMOL )

Software :

For those who need help on Software Installation, please bring your laptop to HFNMRC, B2, IBMS during Day1.

Instructors :

  • Dr. Chi-Fon Chang, GRC, Academia Sinica
  • Ms. Pei-Ju Fang, HFNMRC, Academia Sinica
  • Ms. Yi-Ping Huang, GRC, Academia Sinica
  • Dr. Kuen-Phon Wu, IBC, Academia Sinica
  • Ms. Ellen Tsun-Ai Yu, HFNMRC, Academia Sinica

LAB TAs :

  • Dr. Chi-Fon Chang, GRC, Academia Sinica
  • Ms. Pei-Ju Fang, HFNMRC, Academia Sinica
  • Ms. Yi-Ping Huang, GRC, Academia Sinica
  • Dr. Yuan-Chao Lou, BioTReC, Academia Sinica
  • Dr. Alice Wei, Alumni-IBMS, Academia Sinica
  • Ms. Ellen Tsun-Ai Yu, HFNMRC, Academia Sinica

Tentative Program :

Day 1 : Jan. 27 (Wednesday)
Introduction & Lectures - in English
( Place: B1B/B1C Lecture Room, IBMS, Academia Sinica )
10:00-10:20 Opening & Introductory Overview
by Dr. Chinpan Chen & Dr. Chi-Fon Chang, Academia Sinica
10:20-10:50 NMR Approach for Protein Studies
by Dr. Chi-Fon Chang, GRC, Academia Sinica
10:50-11:10 Coffee Break
11:10-12:00 Protein Production and Labeling
by Ms. Ellen Tsun-Ai Yu, IBMS, Academia Sinica
12:00-12:30 Basic NMR Operation & TopSpin Overview
by Dr. Chi-Fon Chang, GRC, Academia Sinica
12:30-14:00 Lunch Break
( 開放B1A Meeting Room，但建議回各自實驗室或其他可保持合適距離空間用餐。 )
Data Collection 14:00~ (in groups)
( Place: @ HFNMRC, B2 IBMS / @ GRC NMR LAB, Academia Sinica )
  • 90 degree pulse and O1 optimization
  • 1D 1H solvent suppression
  • 2D 15N-HSQC & 13C-HSQC Experiments
  • Set up 3D Experiments for Protein Assignment
Day 2 : Jan. 28 (Thursday)
( Place: B1A/B1C, IBMS, Academia Sinica )
Practical Session I (Topspin) - in English
10:00-10:50 Data Processing using Topspin (Part I)
by Dr. Chi-Fon Chang, GRC, Academia Sinica
10:50-11:10 Coffee Break
11:10-12:00 Data Processing using Topspin (Part II)
by Dr. Chi-Fon Chang, GRC, Academia Sinica
12:00-13:30 Lunch Break
Practical Session II (Sparky) - in Chinese
13:30-15:00 Protein Resonance Assignment using Sparky (Part I)
by Ms. Yi-Ping Huang, GRC, Academia Sinica
15:00-15:20 Coffee Break
15:20 ~ Protein Resonance Assignment using Sparky (Part II)
by Ms. Yi-Ping Huang, GRC, Academia Sinica
Day 3 : Jan. 29 (Friday)
( Place: B1A/B1C , IBMS, Academia Sinica )
Practical Session III (CYANA & MOLOL) - in Chinese
10:00-10:50 Structure Calculation using CYANA (Part I)
by Ms. Pei-Ju Fang, IBMS, Academia Sinica
10:50-11:10 Coffee Break
11:10-11:30 Structure Display using MOLMOL
by Ms. Ellen Tsun-Ai Yu, IBMS, Academia Sinica
11:30-12:00 Structure Calculation using CYANA (Part II)
by Ms. Pei-Ju Fang, IBMS, Academia Sinica
12:00-13:30 Lunch Break
Practical Session IV (PyMOL) - in English
( Place: B1A/B1C, IBMS, Academia Sinica )
13:30-14:20 More on Structure Visualization Program- PyMoL
by Dr. Kuen-Phon Wu, IBC, Academia Sinica
14:20 ~ Q & A

Organizer :

  • HFNMRC, Academia Sinica
  • NMR Lab, GRC, Academia Sinica

Contact Information :

高磁場核磁共振中心
中央研究院 生物醫學科學研究所
11529 台北市南港區研究院路二段128號
HFNMRC
Institute of Biomedical Sciences
Academia Sinica
128, Sec. 2, Academia Rd., Nankang, Taipei 11529, Taiwan R.O.C.
Phone : +886-2-27899047
Fax : +886-2-27887641
Email :
WWW : http://www.nmr.sinica.edu.tw/

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