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2019 NMR Users Workshop
Steps for Protein NMR Structure Determination
High-Field NMR Center & GRC NMR LAB
Academia Sinica
Aug. 14-16, 2019
Time August 14th - 16th, 2019
Registration Deadline August 08th, 2019 (Thursday) ... 逾期恕不受理,敬請配合 !!
Registration Link Registration ... over Deadline
Registration fee Free of charge
Place B1A Meeting Romm & B1B Lecture Room & B2 HFNMRC, IBMS, Academia Sinica, Taipei, Taiwan R.O.C.
Course Language Chinese (Handout in English/Chinese) 中文解說為主
Attach Files

Topic :

  • Useful Experiments for Protein NMR
  • Protein Production and Labeling
  • NMR Data Collection & Processing using Topspin
  • Protein Resonance Assignment using Sparky
  • Protein Structure Calculation using CYANA
  • Protein Structure Display using MOLMOL

Hands-On :

ps: 課程進行中將不支援軟體安裝等協助以避免影響其他學員的學習進度與時間。
  • NMR Data Collection (in groups, 分組進行)
  • NMR Data Processing (事前請先完成安裝Topspin)
  • Protein Backbone Assignment (事前請先完成安裝Sparky)
  • Protein Structure Calculation (事前請先完成安裝Molmol及PuTTY)

Software :

For those who need help on Software Installation, please bring your laptop to HFNMRC, B2, IBMS during Day1.

Instructors / TAs :

  • Dr. Chi-Fon Chang, GRC, Academia Sinica
  • Ms. Pei-Ju Fang, HFNMRC, Academia Sinica
  • Ms. Yi-Ping Huang, GRC, Academia Sinica
  • Dr. Yuan-Chao Lou, CHEM, Academia Sinica
  • Ms. Ellen Tsun-Ai Yu, HFNMRC, Academia Sinica

Tentative Program :

Day 1 : Aug. 14 (Wednesday)
Introduction & Lectures
( Place: B1B Lecture Room, IBMS, Academia Sinica )
10:00-10:10 Opening & HFNMRC Overview
by Dr. Chinpan Chen & Dr. Chi-Fon Chang, Academia Sinica
10:10-10:50 Introduction to NMR Approach for Protein Studies
by Dr. Chi-Fon Chang, GRC, Academia Sinica
10:50-11:10 Coffee Break ( sponsored by Rezwave Co. )
11:10-12:00 Protein Production and Labeling
by Ms. Ellen Tsun-Ai Yu, IBMS, Academia Sinica
12:00-13:30 Lunch Break ( 開放 B1A Meeting Room 用餐 )
13:30-14:00 Basic NMR Operation using TopSpin
by Dr. Chi-Fon Chang, GRC, Academia Sinica
Data Collection 14:00~ (in groups)
( Place: @ HFNMRC, B2 IBMS / @ GRC NMR LAB, Academia Sinica )
  • 90 degree pulse and O1 optimization
  • 1D 1H solvent suppression
  • 2D 15N-HSQC & 13C-HSQC Experiments
  • Set up 3D Experiments for Protein Assignment
Day 2 : Aug. 15 (Thursday)
( Place: B1A Meeting Room, IBMS, Academia Sinica )
Practical Session I (Topspin)
10:00-10:50 Data Processing using Topspin (Part I)
by Dr. Chi-Fon Chang, GRC, Academia Sinica
10:50-11:10 Coffee Break
11:10-12:00 Data Processing using Topspin (Part II)
by Dr. Chi-Fon Chang, GRC, Academia Sinica
12:00-13:30 Lunch Break ( 開放 B1A Meeting Room 用餐 )
Practical Session II (Sparky)
13:30 ~ Protein Resonance Assignment using Sparky
by Ms. Yi-Ping Huang, GRC, Academia Sinica
Day 3 : Aug. 16 (Friday)
( Place: B1A Meeting Room, IBMS, Academia Sinica )
Practical Session III (CYANA & MOLOL)
10:00-10:50 Structure Calculation using CYANA (Part I)
by Ms. Pei-Ju Fang, IBMS, Academia Sinica
10:50-11:10 Coffee Break
11:10-11:30 Structure Display using MOLMOL
by Ms. Ellen Tsun-Ai Yu, IBMS, Academia Sinica
11:30-12:00 Structure Calculation using CYANA (Part II)
by Ms. Pei-Ju Fang, IBMS, Academia Sinica
12:00-13:30 Lunch Break ( 開放 B1A Meeting Room 用餐 )
Group Discussion
( Place: B1B Lecture Room, IBMS, Academia Sinica )
13:30 ~ Q & A

Organizer :

  • HFNMRC, Academia Sinica
  • NMR Lab, GRC, Academia Sinica

Contact Information :

高磁場核磁共振中心
中央研究院 生物醫學科學研究所
11529 台北市南港區研究院路二段128號
HFNMRC
Institute of Biomedical Sciences
Academia Sinica
128, Sec. 2, Academia Rd., Nankang, Taipei 11529, Taiwan R.O.C.
Phone : +886-2-27899047
Fax : +886-2-27887641
Email :
WWW : http://www.nmr.sinica.edu.tw/



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