Magnet_BodyHigh Field NMR Center
Spectrum
Chi-Fon Chang
Name : Chi-Fon Chang

Genomics Research Center, Academia Sinica

No. 128, Sec. 2, Academy Road, Taipei, Taiwan

Tel: (886) 2 2787 1236 / 2789 9157

Fax: (886) 2 2788 7641

E-mail: chifon@gate.sinica.edu.tw


Last Updated : 3 June 2015

Education

  • Ph.D. ( Biophysics), The Ohio State University, U.S.A.
  • B.S. ( Physics), Fu-Jen Catholic University, Taiwan

Professional experiences

  • 2010-current Senior Research Specialist, Genomics Research Center, Academia Sinica
    中央研究院基因體中心 研究技師
  • 2004-2010 Associate Research Specialist, Genomics Research Center, Academia Sinica
    中央研究院基因體中心 研究副技師
  • 2002-2004 High Field NMR Facility Manager, NRPGM, Academia Sinica
    中研院高磁場核磁共振核心 經理
  • 1999-2002 NMR Facility Manager, Institute of Biomedical Sciences, Academia Sinica
    中央研究院生醫所核磁共振實驗室 經理
  • 1998-2002 Adjunct Assistant Professor, Department of Physics Fu-Jen Catholic University
    輔仁大學物理物理系 兼任助理教授
  • 1997-1999 Postdoctoral Fellow, Institute of Biomedical Sciences, Academia Sinica
    中央研究院生醫所 博士後研究員
  • 1989-1990 Research Assistant, Institute of Atomic and Molecular Sciences, Academia Sinica
    中央研究院原分所 研究助理

Research fields

  • NMR Methodology
  • Macromolecular NMR and its applications to Protein Structure/Dynamic/Function

Selected Publications

Journal Articles | Conference Abstracts | Dissertation
    Journal Articles
  • Lo CJ, Chyan CL, Chen YC,Chang CF, Huang HB, Lin TH, 2015, “Resonance assignments and secondary structure of apolipoprotein E C-terminal domain in DHPC micelles.”, Biomolecular NMR assignments, 9(1), 187-190. (SCI) (IF: 0.82; SCI ranking: 92.6%)
  • Polshakov VI1*, Eliseev BD, Frolova LY, Chang CF, Huang TH, 2015, “Backbone 1H, 13C and 15N resonance assignments of the human eukaryotic release factor eRF1”, Biomolecular NMR Assignment, 9(1), 37-42. (SCI) (IF: 0.82; SCI ranking: 92.6%)
  • Huang SY, Chang CF, Fang PJ, Naik MT, Güntert P, Shih HM, Huang TH., 2015, “The RING domain of human promyelocytic leukemia protein (PML).”, J Biomol NMR, 61(2), 173-180. (SCI) (IF: 3.305; SCI ranking: 38.8%)
  • Laurent Sabater, Pei-Ju Fang,Chi-Fon Chang,Aurore De Rache, Enora Prado, Jerôme Dejeu,Antonio Garofalo, Jung-Hsin Lin, Jean-Louis Mergny,Eric Defrancq and Geneviève Pratviel, 2015, “Cobalt(III)porphyrin to target G-quadruplex DNA”, Dalton Transactions, 44, 3701-3707. (SCI) (IF: 4.097; SCI ranking: 15.8%)
  • Huang SY, Naik MT, Chang CF, Fang PJ, Wang YH, Shih HM, Huang TH, 2014, “The B-box 1 dimer of human promyelocytic leukemia protein.”, Journal of biomolecular NMR, 60(4), 275-281. (SCI) (IF: 3.305; SCI ranking: 38.8%)
  • Lo CJ, Wang CC, Huang HB, Chang CF, Shiao MS, Chen YC, Lin TH, 2014, “The Arctic mutation accelerates Abeta aggregation in SDS through reducing the helical propensity of residues 15-25.”, Amyloid : the international journal of experimental and clinical investigation : the official journal of the International Society of Amyloidosis, 1-11. (SCI) (IF: 2.51; SCI ranking: 60.5%)
  • Yi-Chuan Li, Chung-ke Chang, Chi-Fon Chang, Ya-Hsin Cheng, Pei-Ju Fang, Tsunai Yu, Sheng-Chia Chen, Chwan-Deng Hsiao*, Tai-huang Huang*, 2014, “Structural dynamics of the two-component response regulator RstA in recognition of promoter DNA element ”, NUCLEIC ACIDS RESEARCH, 42(13), 8777-8788. (SCI) (IF: 8.808; SCI ranking: 8%)
  • Kung CC, Naik MT, Wang SH, Shih HM, Chang CC, Lin LY, Chen CL, Ma C, Chang CF, Huang TH., 2014, “Structural Analysis of poly-SUMO Chain Recognition by RNF4-SIMs Domain.”, BIOCHEMICAL JOURNAL, 462, 53-65. (SCI) (IF: 4.779; SCI ranking: 21.4%)
  • Chung-ke Chang, Ming-Hon Hou, Chi-Fon Chang, Chwan-Deng Hsiao, Tai-huang Huang*, 2014, “The SARS coronavirus nucleocapsid protein – Forms and functions”, ANTIVIRAL RESEARCH, 103, 39-50. (SCI) (IF: 3.434; SCI ranking: 25.2%)
  • Alan Yueh-Luen Lee, Yu-Da Chen, Yu-Yung Chang, Yu-Ching Lin, Chi-Fon Chang, Shing-Jong Huang, Shih-Hsiung Wu* and Chun-Hua Hsu*, 2014, “Structural basis for DNA-mediated allosteric regulation facilitated by AAA+ module of Lon protease”, ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 70(pt2), 218-230. (SCI) (IF: 7.232; SCI ranking: 6.4%)
  • Ming-Yuan Su, Chung-I Chang and Chi-Fon Chang* , 2013, “1H, 13C and 15N resonance assignments of the pyrin domain from human PYNOD ”, Biomolecular NMR Assignments, 7(2), 141-143. (SCI) (IF: 0.82; SCI ranking: 92.6%)
  • Ming-Yuan Su, Chiao-I Kuo, Chi-Fon Chang*, Chung-I Chang*, 2013, “Three-Dimensional Structure of Human NLRP10/PYNOD Pyrin Domain Reveals a Homotypic Interaction Site Distinct from Its Mouse Homologue”, PLoS One, 8(7), e67843. (SCI) (IF: 3.534; SCI ranking: 18.6%)
  • Wang YS, Ko CH, Chang HT, Yang KJ, Chen YJ, Huang SJ, Fang PJ, Chang CF, Tzou DL.*, 2013, “1H, 13C and 15N backbone and side-chain resonance assignments of a family 36 carbohydrate binding module of xylanase from Paenibacillus campinasensis.”, Biomol NMR Assignment, publish on-line. (SCI) (IF: 0.82; SCI ranking: 92.6%)
  • Chen Hsu, Hsiao-Wen Ho, Chi-Fon Chang, Shang-Ta Wang, Ting-Fang Fang, Min-Hsiung Lee, Nan-Wei Su*, 2013, “Soy isoflavone-phosphate conjugates derived by cultivating Bacillus subtilis var. natto BCRC 80517 with isoflavone ”, FOOD RESEARCH INTERNATIONAL, 53(1), 487-495. (SCI) (IF: 3.05; SCI ranking: 11.8%)
  • Chang CK, Chiang MH, Toh EK, Chang CF, Huang TH*, 2013, “Molecular mechanism of oxidation-induced TDP-43 RRM1 aggregation and loss of function”, FEBS LETTERS, 587(6), 575-582. (SCI) (IF: 3.341; SCI ranking: 38.4%)
  • Winny Ariesandi, Chi-Fon Chang, Tseng-Erh Chen, and Yun-Ru Chen*, 2013, “Temperature-Dependent Structural Changes of Parkinson's Alpha-Synuclein Reveal the Role of Pre-Existing Oligomers in Alpha-Synuclein Fibrillization”, PLoS One, 8(1), e53487. (SCI) (IF: 3.534; SCI ranking: 18.6%)
  • Chun-Hua Hsu* , Tang-Long Shen, Chi-Fon Chang, Yu-Yung Chang, Lin-Ya Huang , 2012, “Solution Structure of the Oncogenic MIEN1 Protein Reveals a Thioredoxin-Like Fold with a Redox-Active Motif”, PLoS One, 7(12), e52292. (SCI) (IF: 3.534; SCI ranking: 18.6%)
  • Sheng-Chia Chen, Chi-Fon Chang, Pei-Ju Fan, Ya-Hsin Cheng,Tsunai Yu, Tai-huang Huang, 2012, “1H, 13C and 15N resonance assignments of the C-terminal DNA-binding domain of RstA protein from Klebsiella pneumoniae”, Biomolecular NMR Assignments, 7(1), 85-88. (SCI) (IF: 0.82; SCI ranking: 92.6%)
  • Ching-Yu Chou, Minglee Chu, Chi-Fon Chang, Tai-huang Huang*, 2012, “A compact high-speed mechanical sample shuttle for field-dependent high-resolution solution NMR ”, JOURNAL OF MAGNETIC RESONANCE, 214, 302-308. (SCI) (IF: 2.315; SCI ranking: 50%)
  • M. T. Naik, C. F. Chang, I. C. Kuo, C. C. H. Kung, K. Y. Chua and T.-H Huang, 2008, "Roles of structure and structural dynamics in the antibody recognition of the allergen proteins: A NMR study on Blomia tropicalis major allergen", Structure, 16, 125-136. (SCI)
  • C. F. Chang, H. T. Chou, Y. J. Lin, S. J. Lee, J. L. Chuang, D. T. Chuang and T. H. Huang, 2006, "Structure of the subunit binding domain and dynamics of the di-domain region from the core of human branched chain alpha-ketoacid dehydrogenase complex", J Biol Chem, 281, 28345-28353. (SCI)
  • S. I. Tyukhtenko, A. V. Litvinchuk, C.-F. Chang, J.-F. Shaw, and T.-h. Huang, "Direct NMR Observation of the Michaelis Complex and its Conversion to the Transition State Analog Complex During the Inhibition of E. coli Thioesterase/protease I by Diethyl p-nitrophenyl Phosphate", (submit to JACS, 2002)
  • C.-F. Chang, H.-T. Chou, J. L. Chuang, D.T. Chuang and T.-h. Huang , "Solution structure and dynamics of the lipoic acid-bearing domain of human mitochondrial branched-chain alpha-ketoacid dehydrogenase complex", J. Biol. Chem., 277, 15865-15873, 2002
  • N.-Y. Su, Y.-D. Liao, C.-F. Chang, I. Wang and C. Chen, "1H, 15N and 13C resonance assignments and secondary structure of the liver ribonuclease from bullfrog Rana catesbeiana", J. Biomol. NMR, 20, 189-190, 2001
  • C.-F. Chang, C. Chen, Y.-C. Chen, K. Hom, R.-F. Huang and T.-h. Huang (1998), "The Solution Structure of a Cytotosic Robonuclease from the Oocytes of Rana catesbeiana (Bullfrog)", J. Mol. Biol. ,283, 231-244.
  • C.-F. Chang and M.H. Zehfus (1998), "Effects of Backbone Modification on Helical Peptides: The Reduced Carbonyl Modification", Biopolymers, 46, 181-193
  • C,-F. Chang and M. H. Zehfus (1996), "The Effect of N-Methylation on Helical Peptides", Biopolymers, 40, 609-616
  • C.-F. Chang and M.H. Zehfus (1996), "Effects of N-Methyl Isosteres on Helix Stability", Peptides: Chemistry, Structure and Bioloby, 531
  • Conference Abstracts
  • Ching-Yu Chou, Chi-Fon Chang, Tai-Huang Huang*, 2012, “NMR Field Cycling Approach : A compact high-speed mechanical design and its application”, paper presented at XXVth International Council on Magnetic Resonance in Biological Systems, Lyon Convention Centre, Lyon, France: Institut Pasteur - CNRS, Paris, France, 2012-08-19 ~ 2012-08-24.
  • Ching-Yu Chou, Chi-Fon Chang, Tai-Huang Huang, 2010, “NMR Field Cycling Approach to Biomolecular Dynamics”, paper presented at XXIVth International Conference on Magnetic Resonance in Biological Systems, Cairns, Australia: International Conference on Magnetic Resonance in Biological Systems, 2010-08-22 ~ 2010-08-27.
  • Ching-Yu Chou, Chi-Fon Chang, Tai-huang Huang*, 2009, “Shuttling Device for Field Cycling Experiment on NMR Relaxation Study in Biological Systems”, paper presented at The 3rd Asia-Pacific NMR Symposium, Ramada Plaza Jeju Hotel, Jeju Island: Korean Magnetic Resonance Society, 2009-10-25 ~ 2990-10-28.
  • C.-F. Chang, P.-J. Fang, T-A. Yu, D. T. Chuang, T.-h. Huang, 2008, “NMR studies of interaction between E2 and E1/E3 components in human branched-chain a-ketoacid dehydrogenase complex”, P476,244 pages, paper presented at XXIIIrd International Conference on Magnetic Resonance in Biology Systems, San Diego, CA, USA: ICMRBS, 2008-08-24 ~ 2008-08-29.
  • C.-F. Chang, T.-A. Yu, H.-T. Chou, D. T. Chuang, T.-h. Huang, 2006, “Studies of Protein Domains in the Transacylase Component of Human Mitochondrial Branched-Chain Ketoacide Dehydrogenase”, PP50,118 pages, paper presented at The 11th Symposium on Recent Advances in Biophysics, Taipei, Taiwan: Biophysics Society of R.O.C., 2006-05-24 ~ 2006-05-26.
  • C.-F. Chang, Y.-J. Lin, H.-T. Chou, M. Wynn, D. T. Chuang, T.-h Huang, 2005, “Studies of Protein Domains in the Transacylase Component of Human Mitochondrial Branched-Chain Ketoacide Dehydrogenase”, AP59,159 pages, paper presented at The 1st Asia-Pacific NMR Symposium, Yokohama, Japan: The NMR Society of Japan, 2005-11-08 ~ 2005-11-11.
  • C.-F. Chang, Y.-J. Lin, H.-T. Chou, S.-J. Lee, D. T. Chuang, and T.-h. Huang, 2005, “Structure and Dynamics Characterization of Protein Domains in the Transacylase Component of Human Mitochondrial Branched-Chain alpha-Ketoacid Dehydrogenase”, P93, 140 pages, paper presented at XXI International Conference on Magnetic Resonance in Biology Systems, Hyderabad, India: ICMRBS, 2005-01-16 ~ 2005-01-21.
  • C.-F. Chang, S.-J. Lee, Y.-J. Lin, B.-N. Chen, T.-h. Huang, 2003, “Structure and Backbone Dynamics of Proteins from transacylase component of Human Mitochondrial Branched-Chain alpha-Ketoacid Dehydrogenase”, PS05 pages, paper presented at Forth East Asian Biophysics Symposium, Taipei, Taiwan: Biophysics Society of R.O.C., 2003-11-03 ~ 2003-11-06.
  • C.-F. Chang, H.-T. Chou, J. L. Chuang, D.T. Chuang, T.-h. Huang, 2002, “Structure and Backbone Dynamics of a Lipoyl Domain from Human Mitochondrial Branched-Chain alpha-Ketoacid Dehydrogenase”, PL356, 221 pages, paper presented at XXth International Conference on Magnetic Resonance in Biology Systems, Toronto, Canada: ICMRBS, 2002-08-25 ~ 2002-08-30.
  • C.-F. Chang, H.-T. Chou, J. L. chuang, D. T. Chuang, T.-h. Huang, 2002, “Solution Structure and Dynamics of the Lipoic Acid-bearing Domain of Human Mitochondrial Branched-Chain alpha-Ketoacid Dehydrogenase Complex”, 48 pages, paper presented at The 8th Symposium on Recent Advances in Biophysics, Academia Sinica, Taiwan: Biophysics Soceity of R.O.C., 2002-05-22 ~ 2002-05-25.
  • C.-F. Chang, H.-T. Chou, J. L. Chuang, D.T. Chuang,T.-h. Huang, 2001, “Solution structure of the lipoic acid-bearing domain of human mitochondrial branched-chain alpha-ketoacid dehydrogenase”, POS9-A-16 pages, paper presented at The 9th SCBA International Symposium, Academia Sinica, Taiwan: SCBA, 2001-08-05 ~ 2001-08-10.
  • C.-F. Chang, H.-T. Chou, O. Gorbatyuk, J. Chuang, D. Chaung, T.-h. Huang, 2001, “The Solution Structure of the Lipoic Acid-bearing Domain of Human Mitochondrial Branched-Chain alpha-Ketoacid Dehydrogenase Complex”, P02, 52 pages, paper presented at The 7th Symposium on Recent Advance in Biophysics, Taipei, Taiwan: Biophysics Soceity of R.O.C., 2001-05-23 ~ 2001-05-26.
  • S. I. Tyukhtenko, A. V. Litvinchuk,C.-F. Chang,T.-h. Huang, 2000, “NMR Detection and Characterization of Strong Hydrogen Bonds in the Catalytic Triad of E. coli Thioesterase/protease I”, M&T P052 pages, paper presented at The 42nd Experimental Nuclear Magnetic Resonance Conference, Pacific Grove, California, USA: ENC, 2000-04-09 ~ 2000-04-14.
  • C.-F. Chang, C. Chen, Y.-C. Chen, Y. Wu, K. Hom, T.-h Huang, 1998, “Structure and Function of Cell cytotoxic Ribonuclease by NMR Spectroscopy”, 86 pages, paper presented at 1998 Fall Camp, Enzymes: Function and Structure, Taiwan: The Society for Biochemistry and Molecualr Biology, Taiwan, 1998-10-30 ~ 1998-11-01.
  • C.-F. Chang, C. Chen, Y.-C. Chen, K. Hom, R.-F. Huang,T.-h. Huang, 1998, “Solution Structure and binding studies of RC-RNase by 1H NMR Spectroscopy”, paper presented at XVIIIth International Conference on Magnetic Resonance in Biology Systems, Tokyo, Japan: ICMRBS, 1998-08-23 ~ 1998-08-28.
  • C.-F. Chang, C. Chen, Y.-C. Chen, K. Hom, R.F. Huang,T.-h Huang, 1998, “Solution Structure of the Complexs of RC-RNase with 2',5'-CpG and 3',5'-d(CpG)”, S27 pages, paper presented at The 4th Symposium on Recent Advances in Biophysics, Taipei, Taiwan: Biophysics Society of R.O.C., 1998-05-13 ~ 1998-05-16.
  • C.-F. Chang,M. H. Zehufs, 1997, “The Effect of Peptide Backbone Modification on Helix Structure”, paper presented at Biophysical Society Annual Meeting, New Orleans, Louisiana, USA: Biophysical Society, 1997-02-01 ~ 1997-02-06.
  • C.-F. Chang,M. H. Zehfus, 1996, “The Effect of N-Methyl Isosteres on Helical Peptides”, paper presented at Protein Science Society Meeting, LA, USA: Protein Science Society, 1996-06-07 ~ 1996-06-14.
  • C.-F. Chang,M.H. Zehfus, 1995, “Effects of N-Methyl Isosters on Helix Stability”, paper presented at 14th American Peptide Symposium, Columbus OH, USA: The Ohio State University, USA, 1995.
  • Dissertation (1997, The Ohio State University)
  • "STUDIES IN PROTEIN FOLDING: UNDERSTANDING HELIX BACKBONE INTERACTIONS AND THE STRUCTURAL CHARACTERIZATION OF A COMPACT DOMAIN"
Magnet_Foot
中央研究院。高磁場核磁共振中心
High Field Nuclear Magnetic Resonance Center, Academia Sinica