Login
HFNMRC HOME2024 NMR Workshop
Protein NMR SOP - Assignment & Structure CalculationHigh-Field NMR Center & GRC NMR Lab
Academia Sinica
Academia Sinica
Dec. 4, 2024
| Date | December 04th, 2024 (Wednesday) |
| Time | 10:00 - 17:00 |
| Registration Deadline | November 29th, 2024 (Friday) |
| Registration Link | Registration ... over Deadline |
| Registration fee | Free of charge |
| Place | 1F Auditorium, GRC, Academia Sinica, Taipei, Taiwan R.O.C. |
| Language | Chinse (Mainly in Chinese) |
| Photos | let's view >> ( Last updated : 20241210 ) |
Introduction :
此次研習將簡單介紹利用核磁共振進行蛋白質結構分析的步驟！ 蛋白質相關實驗及軟體琳瑯滿目，本次內容規劃不講深奧原理，以"小老師領進門”的概念進行，分享樣品準備技巧，實驗設計介紹(1H-detection, 13C-detection)，圖譜分析軟體入門，結構計算軟體(與會者可自備筆電，先安裝好軟體，當天跟著講師一起逐步操作)。 希望讓與會者對於利用NMR來決定蛋白質結構的步驟有基本的認識 ~
2024 NMR Workshop is aimed to introduce basic Protein NMR SOP on assignment and structure calculation. We will share experience on sample preparation, useful experiments (1H-detecion, 13C-detection), data analysis software, and structure determination server (participants could bring your labtop, install software in advance, and follow the instructor step by step). Lectures will be in Chinese but handout in English.
Topic :
- Protein Production and Labeling
- Useful Experiments for Protein NMR
- Protein Resonance Assignment using Sparky (POKY)
- Protein Resonance Assignment using CARA
- Automatic Structure Calculation using POKY
- Automation using NMRtist (CYANA)
Software :
(bring your labtop with software installed in advance, and follow the instructor step by step)- Install POKY (Sparky)：https://sites.google.com/view/pokynmr
- Install CARA：http://cara.nmr.ch/doku.php/cara_downloads
- Create a free account in NMRtist：https://nmrtist.org/
Instructors / TAs :
- Dr. Chi-Fon Chang 張七鳳 博士, Genomics Research Center, Academia Sinica
- Ms. Pei-Ju Fang 方珮如, HFNMRC, Academia Sinica
- Ms. Yi-Ping Huang 黃意評, GRC, Academia Sinica
- Ms. Ellen Tsun-Ai Yu 游春愛, HFNMRC, Academia Sinica
Tentative Program :
| 09:40- | Registration |
| 10:00-10:10 | Opening & Introductory Overview by Dr. Chi-Fon Chang, Academia Sinica |
| 10:10-10:30 | Protein Production and Labeling by Ms. Tsun-Ai Yu, Academia Sinica |
| 10:30-10:45 | Break |
| 10:45-11:20 | Useful Experiment for Protein NMR by Dr. Chi-Fon Chang, Academia Sinica |
| 11:20-12:10 | Resonance Assignment using Sparky* by Ms. Yi-Ping Huang, Academia Sinica |
| 12:10-13:30 | Lunch Break |
| 13:30-14:20 | Resonance Assignment using CARA* by Ms. Tsun-Ai Yu, Academia Sinica |
| 14:20-15:10 | Structure Calculation using POKY* by Ms. Pei-Ju Fang, Academia Sinica |
| 15:10-15:30 | Break |
| 15:30-16:00 | Structure Calculation using NMRtist* by Dr. Chi-Fon Chang, Academia Sinica |
| 16:00-17:00 | Discussion & Q.A |
* 建議與會者自備筆電，先安裝好軟體並下載demo data set。 當天跟著講師一起逐步操作！！ 為避免影響課程進度，講師講解基本步驟，但不刻意等待，請儘可能跟上，或自行練習 (當天亦不支援軟體安裝)。
* If you like, bring your labtop and install all software in advance, you can follow the instructor step by step. Due to the time limitation, please try to catch up during the course, or you can practice at home.
Organizer :
- HFNMRC, Academia Sinica
- NMR Lab, GRC, Academia Sinica
Contact Information :
高磁場核磁共振中心
中央研究院 生物醫學科學研究所
11529 台北市南港區研究院路二段128號
中央研究院 生物醫學科學研究所
11529 台北市南港區研究院路二段128號
HFNMRC
Institute of Biomedical Sciences
Academia Sinica
128, Sec. 2, Academia Rd., Nankang, Taipei 11529, Taiwan R.O.C.
Institute of Biomedical Sciences
Academia Sinica
128, Sec. 2, Academia Rd., Nankang, Taipei 11529, Taiwan R.O.C.
| Phone : | +886-2-27899047 |
| Fax : | +886-2-27887641 |
| Email : | hfnmrc@gmail.com |
| WWW : | https://www.nmr.sinica.edu.tw/ |
© 2024 High Field Nuclear Magnetic Resonance Center ( HFNMRC ). All rights reserved.
Best viewed at 1024x768 16bit color resolution or above