Mandar T. Naik, Ph.D.

Postdoctoral Research Associate
    Telephone +886 2 2789 9039
    E-mail mandarn(at)ibms.sinica.edu.tw (at)=@
    Office Rm. N720
    Institute of Biomedical Sciences
    No. 128, Sec. 2, Academy Road
    11529 Taipei, Taiwan.

Field of research


Applied protein NMR spectroscopy, Solution structure and dynamics, Protein: protein / ligand interaction studies.


 

Current Projects:


    We work on the NMR solution structures of various dust mite allergens. We recently solved structure of the major allergen from Blomia Tropicalis mites, Blo t 5 and identified mAb epitope of this important allergen by exclusive use of NMR experiments. We are currently preparing modified Blo t 5 variants, which may have potential to be used in immunotherapy of allergic diseases.

    We also work SUMOlyation of an important transcriptional co-repressor. 

Publications

(Partial list of 6 out of 12).

 

1.   Naik M.T., Chang C-F., Kuo I-C., Kung C.C-H., Yi F-C., Chua K-Y. and Huang T-H. (2007) Roles of structure and structural dynamics in the antibody recognition of the allergen proteins: A NMR study on Blomia tropicalis major allergen, Structure (In press).

 

2.   Naik M.T., Chang C-F., Kuo I-C., Yu T., Fang P-J., Chua K-Y. and Huang T-H. (2007) Complete 1H, 13C and 15N resonance assignments of Blo t 5, a major mite allergen from Blomia tropicalis, Journal of Biomolecular NMR, 38, 189.

 

3.   Naik M.T., Suree N., Ilangovan U., Liew C.K., Thieu W., Campbell D.O., Clemens J.J., Jung M.E., Clubb R.T. (2006) Staphylococcus aureus Sortase-A transpeptidase: Calcium promotes sorting signal binding by altering the mobility and structure of an active loop, Journal of Biological Chemistry, 281, 1817.

 

4.  Naik M.T., Lee H-J., Bracken C. and Breslow E. (2005) NMR investigation of main-chain dynamics of the H80E mutant of bovine neurophysin–I: Demonstration of dimerization-induced changes at the hormone-binding site, Biochemistry, 44, 11766.

 

5.  Naik M.T. and Huang T-H. (2004) Conformational stability and thermodynamic characterization of the lipoic acid bearing domain of human mitochondrial branched chain a-ketoacid dehydrogenase, Protein Science, 13, 2483.

 

6.  Naik M.T., Chang Y-C. and Huang T-H. (2002) Folding kinetics of the lipoic acid bearing domain of human mitochondrial branched chain a-ketoacid dehydrogenase complex, FEBS Letters, 530, 133.