Protein Solution NMR Spectroscopy

Contents

1. Basic NMR theory, including quantum mechanical and vectorial descriptions of NMR spectroscopy.
   
2. Basic experimental aspects of NMR: NMR data acquisition and processing.
   
3. Product operator formalism analysis of pulse programs.
   
4. Spin dynamics: Coherent selection, phase cycling, gradient enhanced spectroscopy.
   
5. Heteronuclear multidimensional NMR spectroscopy.
   
6. Relaxation and protein dynamics.
   
7. Special topics: TROSY, RDC and reduced dimensionality etc.
   
8. Applications in protein NMR in solution.
   

Mail  to Ms. Chen: chin@ibms.sinica.edu.tw

• Topics : Protein Solution NMR Spectroscopy.

• Instructor : Tai-huang Huang, (Chi-Fon Chang and Winston Wu)

• Time :Every Saturday 10:00 AM - 1:00 PM for ~ 15 sessions

              (First session: Oct 2, 2004)

• Place : B1A room, Institute of Biomedical Sciences, Academia Sinica

• Pre-requisite : Some prior experience with protein NMR spectroscopy is required.

Textbooks :

1. Lecture by James Keeler on “Understanding NMR spectroscopy”
   (http://www-keeler.ch.cam.ac.uk/lectures/)
2. Cavanagh, Fairbrother, Palmer, and Skelton: “Protein NMR spectroscopy –
   Principles and practice” Academic press, 1996.
3. Selected review articles.

Documents

1. lecture 1
2. lecture 2
3. lecture 3
4. lecture 4
5. lecture 5
6. lecture 6
7. lecture 6.1: density matrix ppt file, and document
8. lecture 7: 2D NMR
9. lecture 8: Phase cycling
10. lecture 9: Coherence
11. lecture 10: Gradient Pulse
12. lecture 11: Application

Reference

1. Residual dipolar couplings in structure determination of biomolecules.
Prestegard, Bougault, Kishore. Chem Rev 2004, 104, 3519-3540.
2. Residual dipolar couplings in NMR structure analysis.
Litsitz and Tjandra. Ann Rev Biophys Biomol Struct 2004 33, 387-413.
3. Residual dipolar coupling constants: An elementary derivation of key equations.
Kramer, Deshmukh, Kessler, Glaser. Conc in Mag Res 2004 21A(1), 10-21.