Institute of Biomedical Sciences,
Academia Sinica
Nankang, Taipei 11529, Taiwan R.O.C.
Tel: 886-2-2652-3036

886-2-2789-9039

Fax: (886)-2-2788-7641

Update: 3 August 2014

    2014

  • Li, Y.-C., Chang, C.-K., Chang, C.F., Cheng, Y.H., Fang, P.R., Yu, T., Chen, S.C., Hsiao, C.D.* and Huang, T.-h.*(2014)
    "Structural dynamics of two-component response regulator RstA from Klebsiella pneumoniae in recognition of promoter DNA element". Nucleic Acid Res. (Online July 2, 2014; doi: 10.1093/nar/gku572)

  • Kung, C.C.H., Nail, M.T., Wang, S.H. Shih, H.M. Chang, C.C., Lin, L.Y. Chen, Ma, C., Chang, C.-F. and Huang, .T.-h. (August 2014)
    "Structural Analysis of Poly-SUMO Chain Recognition by RNF4-SIMs Domain" Biochem. J. 462(1):53-65. (IF: 4.779. 61/291 in BC & MB) (Online: May 20, 2014; doi:10.1042/BJ20140521)

  • Hsu, Y.R., Kang, Y.W., Fang, J.Y., Lee, G.J., Chyi, J.I. Chang, C.K., Huang, C.C. Hsu, C.P., Huang, T.H., Huang, Y.F., Sun, Y.C., Hsu, C.H., Chen, C.C., Li, S.S. Yeh, J.A., Yao, D.J., Ren, F., & Wang, Y.L. (March 2014)
    "Investigation of C-terminal domain of SARS nucleocapsid protein¡VDuplex DNA interaction using transistors and binding-site models" Sensors and Actuators B: Chemical, 193, 334-339.

  • Polshakov, V.I., Eliseev, B.D., Frolova, L.Y., Chang, C.F., Huang, T.H. (2014)
    "Backbone 1H, 13C and 15N resonance assignments of the human eukaryotic release factor eRF1 Biomolecular NMR assignments" (Online Jan 26, 2014).

  • Chang, C.K., Hou, M. H., Chang, C.F., Hsiao, C.D. and Huang, T.H.* (Jan. 2014)
    "The SARS Coronavirus Nucleocapsid Protein - Forms and Functions" Antiviral Res. 103:39-50. ( NSC 101-2311-B-001-025)

  • 2013

  • Hsueh, K.L. Yu, L.K., Chen, Y.H., Cheng, Y.H., Hsieh, Y.C., Ke, S.C., Hung, K.W., Chen, C.J. and Huang, T.-h.* (Oct. 2013)
    "FeoC from Klebsiella pneumoniae contains a [4Fe-4S] cluster" J. Bacteriol. 195(20): 4726-4734; published ahead of print 16 August 2013 doi:10.1128/JB.00687-13 (NSC100-2311-B-001-023 ).

  • Chang, C.K., Chen, C.M., Chiang, M.H.. Hsu, Y.L., Chang, C.F. and Huang., T.-h.* (May, 2013)
    ”Transient Oligomerization of the SARS-CoV N Protein – Implication for virus ribonucleoprotein packaging”, PLoS ONE 8 (5) e65045, doi: 10.1371/journal.pone.0065045.

  • Chen, I.J., Yuann, J.M., Chang, Y.M., Lin, S.Y., Zhao, Perlman, S., Shen, Y.Y., Huang, T.-h.; Hou, M.H.* (June, 2013)
    ”Crystal structure-based exploration of the important role of Arg106 in the RNA-binding domain” of human coronavirus OC43 nucleocapsid protein” Biochimica et Biophysica Acta – Proteins and Proteomics 1834(6): 1054-1062. Doi: 10.1016/j.bbapap.2013.03.003.

  • Chang, C.K., Chiang, M.H., Toh, E.K.-W., Chang, C.F. and Huang, T.-h.* (March, 2013)
    ”Molecular mechanism of oxidation-induced trans-activating response region DNA-binding protein of 43 kDa (TDP-43) aggregation and loss of function” FEBS Lett. 587 (6), 575-582

  • Hsieh, Y.L., Kuo, H.Y., Naik, M.T., Chang, C.C., Liao, P.H., Ho, C.C., Huang, T.C., Jeng, J.C., Hsu, P.H., Tsai, M.D., Huang, T.-h. and Shih, S.H.* (March 2013)
    ”Ubc9 acetylation modulates distinct SUMO target modification and hypoxia response”. EMBO J. 32, 791-804 (Comment by Andy Sharrocks. Recommended by faculty 1000 http://f1000.com/prime/717979528)

  • Chen, S.C., Chang, C.F., Fan, P.J., Cheng, Y.H., Yu, T. and Huang, T.-h.* (March, 2013)
    ”1H, 13C and 15N resonance assignments of the C-terminal DNA-binding domain of RstA protein from Klebsiella pneumoniae” Biomol. NMR Assign. 7, 85-88.

  • Lo, Y.S., Lin, S.Y., Wang, S.M., Wang, C.T., Chiu, Y.L., Huang, T.-h. and Hou, M.H.* (Jan, 2013)
    ”Oligomerization of the carboxyl terminal domain of the human coronavirus 229E nucleocapsid” FEBS Lett. 587 (2), 120-127.

  • 2012

  • Hung, K.W., Tsai, J.Y., Juan, T.H., Hsu, Y.L., Hsiao, C.D.* and Huang, T.H.* (Dec, 2012)
    ”Crystal structure of KpNFeoB/KpFeoC complex and the roles of FeoC in the regulation of Fe2+ transport by the bacterial Feo system”. J. Bacteriology, 194(23), 6518-26.

  • Chang, C.K., Wu, T.H., Wu, C.Y., Chiang, M.H., Toh, E.K.W., Hsu, Y.C., Lin, K.F., Liao, Y.H., Huang, T.-h*., Huang, J.J.T.* (Aug, 2012)
    ”The effect of the N-terminus on the oligomerization process and DNA binding affinity of TDP-43” Biochem. Biophys. Res. Comm. 425 (2), 219-224.

  • Hung, K.W., Juan, T.H., Hsu, Y.L. and Huang, T.-h.* (June, 2012)
    NMR structure note: The ferrous iron transport protein C (FeoC) from Klebsiella pneumoniae, J. Biomol. NMR 53(2), 161-165.

  • Sekiyama, N., Jee, J., Isogai, S., Akagi, K.I., Huang, T.-h., Ariyoshi, M., Tochio, H. and Shirakawa, M.*
    NMR analysis of Lys63-linked polyubiquitin recognition by the tandem ubiquitin-interacting motifs of Rap80. J Biomol NMR. 52(4), 339-350.(2012)

  • Chou, C.Y., Chu, M., Chang,C.F., and Huang, T.H.*
    A compact high-speed mechanical sample shuttle for field-dependent high-resolution solution NMR, J. Magn. Res., 214, 302-308 (2012)

  • 2011

  • Chang, C.C., Naik, M.T., Huang, Y.S., Jeng, J.C., Liao, P.H., Kuo, H.Y., Ho, C.C., Hsieh, Y.L., Lin, C.H., Huang, N.J., Naik, N.M., Kung, C.C.H., Lin, S.Y., Chen, R.H., Chang, K.S., Huang, T.H.*, and Shih, H.M.*
    Structural and functional roles of Daxx SIM phosphorylation in SUMO paralogue-selective binding and apoptosis modulation, Mol. Cell, 42, 62-74 (2011)

  • Brautigam*, C. A. Wynn, R. M., Chuang, J. L., Naik, M.T., Young, B.B., Huang, T.H. and Chuang, D.T.*
    Structural and Thermodynamic Basis for Weak Interactions between Dihydrolipoamide Dehydrogenase and Subunit-binding Domain of the Branched-chain α-Ketoacid Dehydrogenase Complex, J. Biol. Chem. 286: 23476- 23488 (2011)

  • Jiang, I., Tsai, C.-K., Chen, S.C., Wang, S., Amiraslanov, I., Chang, C.F., Wu, W.J., Tai, J.H., Liaw, Y.C.* and Huang, T.-H*.
    Molecular basis of the recognition of the multifarious transcription promoter elements of ap65-1 gene by Myb2 from the protozoan parasite Trichomonas vaginalis, Nucleic Acid Res. Published online July 19, 2011. (Online July 19, 2011)

  • 2010

  • Hung, K.W., Chang, Y.W., Eng, E.T., Chen, J.H., Chen, Y.C., Sun, Y.J., Hsiao, C.D.*, Dong, G., Spasov, K.A., Unger, V.M., and Huang, T.-h.*
    Structural fold, conservation and Fe(II) binding of the intracellular domain of prokaryote FeoB, J. Struct. Biol. 170, 501-512 (2010)

  • Wang, C.H., Davamani, F., Sue, S.C., Lee, S.C., Wu, P.L.., Tang, F.M., Shih, C., Huang, T.-h.* and Wu*, W.G.
    Cell surface heparan sulfates mediate internalization of PWWP/HATH domain of HDGF via macropinocytosis to fine-tune cell signaling process involved in fibroblast cell migration, Biochem. J. 433, 127-138.(2010)

  • Naik, M.N., Chang, C.C., Naik, N., Kung, C.C-H, Shih, H.-M. and Huang, T.H.*
    NMR chemical shift assignments of a complex between SUMO-1 and SIM peptide derived from the C-terminus of Daxx, Biomol. NMR Assign. 5, 75-77(2010)

  • 2009

  • Fang, H. J., Chen, Y. Z., Li, M. S.*, Wu, M. C.*, Chang, C. L., Chang, C. K., Hsu, Y. L., Huang, T. -h.*, Chen, H. M., Tsong, T. Y.*, Hu, C. K.*
    Thermostability of the N-terminal RNA-binding domain of the SARS-CoV nucleocapsid protein: Experiments and numerical simulations, Biophys. J. 95(5), 1892-1901 (2009)

  • Chang, C.K., Hsu, Y.L., Chang, Y.H., Chao, F.A., Wu, M.C., Huang, Y.S., Hu, C.K., Huang, T.-h.*
    Multiple Nucleic Acid Binding Sites and Intrinsic Disorder of SARS Coronavirus Nucleocapsid Protein - Implication for Ribonucleocapsid Protein Packaging, J. Virol. 83, 2255-2264 (2009)

  • 2008

  • Naik, M.T., Chang, C.F., Kuo, I.C., Kung, C.C.H., Chua, K.Y.* and Huang, T.-h.*
    Roles of structure and structural dynamics in immune system recognition of allergen protein: A NMR study on Blomia tropicalis major allergen, Structure 16, 125-136 (2008)

  • Takeda, M., Chang, C.K., Ikeya, T. Güntert, P., Chang, Y.S., Hsu, Y.L., Huang, T.-h.*, and Kainosho, M.*
    Solution Structure of the C-terminal Dimerization Domain of SARS Coronavirus Nucleocapsid Protein Solved by the SAIL-NMR Method, J. Mol. Biol. 380, 608-622 (2008)

  • 2007

  • Chen, C.Y., Chang, C.K., Chang, Y.W., Sue, S.C., Bai, H.I., Riang, L., Hsiao*, C.D., Huang, T.-h.*
    Structural insights into packaging of the helical nucleocapsid from the crystal structure of SARS coronavirus nucleocapsid protein dimerization domain, J. Mol. Biol. 368, 1075-1086. (2007)

  • Sue, S.C., Lee, W.T., Tien, S.C., Yu, J.G., Lee, S.C., Wu, W.J., Wu*, W.G. and Huang, T.-h.*
    PWWP module of human hepatoma-derived growth factor forms a domain-swapped dimer with much higher affinity for heparin. J. Mol. Biol. 367, 456–472 (2007)

  • Mandar T. Naik, and Huang, T.-h.*
    Complete 1H, 13C and 15N resonance assignments of Blo t 5, a major mite allergen from Blomia tropicalis. J. Biomol NMR 38, 189. (2007)

  • 2006

  • Chang, C.F., Chou, H.T., Lin, Y.J., Lee, S.J., Chuang, J.L., Chuang,, D.T. and Huang, T.-h.*
    Structure of the Subunit Binding Domain and Dynamics of the “Di-domain” Region from the Core of Human Branched-Chain α-Ketoacid Dehydrogenase Complex J. Biol. Chem. 281 (38), 28345-53. (2006)

  • W.-J. Wu and T.-h. Huang*(2006)
    NMR in Drug Discovery教育部顧問室醫藥基因生物技術教學資源中心.

  • Wu, W.J., Tyukhtenko, S.I. and Huang, T.-h.
    Direct NMR Resonance Assignments of the Active Site Histidine Residue in Escherichia coli Thioesterase I/Protease I/Lysophospholipase L1. Magnetic Resonance in Chemistry, 44, 1037-1040 (2006)

  • Kuo-Wei Hung, Yi-Chao Lin, Chun-Chia Cheng, Pei-Ju Fan, Chi-Fon Chang, Shih-Feng Tsai and Tai-Huang Huang
    Resonance assignments of a CoA binding protein from Klebsiella pneumoniae J. Biomol. NMR 36, 74. (2006)

  • Chang, C.K., Sue, S.C., Yu, T.H., Hsieh, C.M., Tsai, C.K., Chiang, Y.C., Lee, S.J., Hsiao, H.H., Wu, W.J., Chang, W.L., Lin, C.H. and Huang, T.-h.*
    Modular organization of SARS coronavirus nucleocapsid protein. J. Biomed. Sci. 13, 59-72. (2006)

  • Wu, K.P., Chang, J.M, Chen, J.B., Chang, C.F., Wu, W.J., Huang, T.-h*., Sung, T.Y., Hsu, W.L.*
    RIBRA-an Error-Tolerant Algorithm for the NMR Backbone Assignment Problem. J. Comp. Biol. 13, 229-244. (2006)

  • Sue, S.C., Hsiao, H.H., Chung, C.P.B., Cheng, Y.H., Hsueh, K.L., Chen, C.M., Ho, C.H. and Huang, T.-h.*
    Solution structure of the DNA binding domain of the telomeric repeat-binding protein of Arabidopsis thaliana: A new fold with an additional C-terminal helix J. Mol. Biol. 356, 72-85. (2006)

  • 2005

  • Chang, C.K., Sue, S.C., Yu, T.H., Hsieh, C.M., Tsai, C.K., Chiang, Y.C., Lee, S.J., Hsiao, H.H., Wu, W.J., Chang, C.F. and Huang, T.-h.*
    The dimer interface of the SARS coronavirus nucleocapsid protein adapts a porcine respiratory and reproductive syndrome virus-like structure FEBS Lett. 579, 5663-5668. (2005)

  • Hsieh, P.K., Chang, S.C., Chen, I.Y., Kou, Y.H., Chang, C.K. Huang, T.-h., and Ming-Fu Chang
    The Assembly of an RNA Packaging Signal Containing Virus-Like Particle of SARS-CoV is Nucleocapsid-dependent J. Virol. 79, 13848-13855. (2005)

  • Kouza, M., Chang, C.-F., Hayryan, S., Yu, T.H., Li, M.S., Huang, T.-h.* and Hu, C.K*.
    Folding of the protein domain hbSBD. Biophys. J. 89, 3353-3361. (2005)

  • Sue, S.C., Chang, C.F., Huang, Y.T., Chou, C.Y. and Huang, T.-h.*
    Challenges in NMR-based Structural Genomics. Physica A 350, 12-27 . (2005)