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日期:107年7月24日至8月9日
時間:每週二至週四 下午兩點至五點
第一週 7/24-7/26
第二週 7/31-8/2
第三週 8/7-8/9
地點:台灣大學化學系121教室
聯絡人:徐尚德 02-27855696 ext 5120 | sthsu@gate.sinica.edu.tw
授課講師:- 陳振中教授(台灣大學化學系)
- 余慈顏博士(中央研究院原子與分子研究所)
- 徐尚德博士(中央研究院生物化學研究所)
報名網址 : click here
註冊期限:107年7月1日
課程大綱:- Introduction of NMR hardware designs
- Signal detection and Fourier transformation - Practical aspects of (solution state) NMR data acquisitions
- Data acquition parameters - solvent type, pulse calibration, solvent suppression, relationship between spectral width, data points and acquisition time, decoupling, temperature effect, etc.
- Data processing (hands-on practice with Topspin) window function, zero filling, linear prediction, solvent subtraction, baseline correction, etc. - Theoretical aspect of NMR spectroscopy
- Product operator formalism
- NMR relaxation dynamics and lineshape
- Principles of multidimensional NMR - Introduction of common solution state NMR experiments
- 1D 1H and 13C NMR
- 2D homonuclear NOESY, COSY and TOCSY
- 2D heteronuclear 13C/15N HSQC, HMQC and HMBC
- Diffusion order spectroscopy (DOSY) - Applications of NMR in structural biology, medicinal chemistry and material sciences
- Protein NMR spectroscopy, assignment and isotope-labeling
- Molecular interaction mapping by NMR - Measurements and analysis of solid state NMR spectra under magic angle spinning
參考書目:click here |